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Image Search Results
Journal: Stem Cells International
Article Title: Production of Adult Human Synovial Fluid-Derived Mesenchymal Stem Cells in Stirred-Suspension Culture
doi: 10.1155/2018/8431053
Figure Lengend Snippet: Multipotent differentiation potential of SF-MSCs cultured for 18 days in spinner flasks using the bead-to-bead transfer method. Multipotency was evaluated for cells isolated from both donor 1 and donor 2. Shown here are photomicrographs for donor 1, which are representative of the qualitative results obtained for donor 2. (a) Osteogenic differentiation of donor 1 SF-MSCs. Calcium deposition is an indication of osteogenesis and was detected using Alizarin Red. Photomicrograph was taken at 10x magnification. Scale bar represents 200 μ m. (b) Adipogenic differentiation of donor 1 SF-MSCs. Intracellular lipid droplet formation is an indication of adipogenesis and was detected using Oil Red O. Photomicrograph was taken at 20x magnification. Scale bar represents 100 μ m. (c) Chondrogenic differentiation of donor 1. Glycosaminoglycan (GAG) production is an indication of chondrogenesis and results in an increase in pellet size (right pellet was induced; left pellet was not induced (control)). Photomicrograph was taken at 5x magnification. Scale bar represents 500 μ m. (d) Quantitative chondrogenic differentiation analysis of donor 1 and donor 2 using the pellet culture method. 2.5 × 10 5 cells/pellet were cultured in either 10% FBS DMEM (control) or chondrogenic induction medium (induced) for 28 days at 37°C and 5% CO 2 . GAG production was quantified using 1,9-dimethylmethlylene blue after digesting the pellets in papain solution. Data were collected in triplicate; error bars represent the standard deviation of the data. ∗ p < 0.05 compared to the control.
Article Snippet: Cryopreserved SF-MSCs at passage 2 were thawed and inoculated into 75 cm 2 Nunc tissue culture flasks (T-75) at a density of 5000 cells/cm 2 with
Techniques: Cell Culture, Isolation, Control, Standard Deviation